α-Synuclein Attenuates Maneb Neurotoxicity through the Modulation of Redox-Sensitive Transcription Factors

Figure 5

NFR2 pathway is downregulated in cells with endogenous α-synuclein upon MB exposure. (a, c) Immunocytochemistry analysis reveals NRF2 and BACH1 nuclear expression in control and wt α-syn cells exposed to the pesticide. Hoechst was used as nuclear marker. Fluorescence intensity quantification was assessed with ImageJ. Results are expressed as arbitrary units. The pictures shown are representative of at least three different experiments. Scale bars: 20 μm. (b) mRNA levels of GCLc and HO-1, both NRF2 downstream genes, were determined in cells with endogenous and overexpressed α-synuclein in the presence of MB. mRNA expression was assessed by qRT-PCR. Results are expressed as fold change compared to the control and represent (). (a–c) for each condition with respect to the control; one-way ANOVA and Tukey’s post hoc test.

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