α-Synuclein Attenuates Maneb Neurotoxicity through the Modulation of Redox-Sensitive Transcription Factors

Figure 6

α-Synuclein modulates FOXO3a pathway leading to SOD2 upregulation in wt α-syn cells after MB treatment. (a) FOXO3a expression and cellular localization were evaluated by immunocytochemistry studies in cells with endogenous levels and overexpressing α-synuclein (control and wt α-syn cells, respectively) exposed to the pesticide (24 μM) or vehicle for 48 h. Hoechst was used as nuclear marker. Results are expressed as arbitrary units, and ImageJ was used for fluorescence intensity quantification. Scale bar: 20 μm. (b) SOD2 expression in control and wt α-syn cells was analyzed by Western blot in total fractions after MB exposure. α-Tubulin was used as loading control. One representative blot of three different experiments is shown. Bands of proteins were quantified using ImageJ. Results are expressed as arbitrary units. (c) Catalase activity was measured in control and wt α-syn cells after MB exposure. Results are expressed as arbitrary units per mg of protein. (a–c) , and with respect to the corresponding control; one-way ANOVA and Tukey's post hoc test. Results represent mean.

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