α-Synuclein Attenuates Maneb Neurotoxicity through the Modulation of Redox-Sensitive Transcription Factors

Figure 7

Effect of MB exposure on FOXO3a phosphorylation by the PI3K-AKT pathway in control and wt α-syn cells. (a) Immunocytochemistry studies show the expression of phosphorylated AKT in control and wt α-syn cells exposed to MB. (b) Nuclear FOXO3a levels were determined by immunocytochemistry under the inhibition of PI3K/AKT pathway using 10 μM LY294002 in the presence of MB. (a, b) Hoechst was used as nuclear marker. Fluorescence intensity was quantified using ImageJ. Results are expressed as arbitrary units. for each condition with respect to the control; one-way ANOVA and Tukey’s post hoc test. Scale bars: 20 μm.

(a)

(b)