α-Synuclein Attenuates Maneb Neurotoxicity through the Modulation of Redox-Sensitive Transcription Factors

Figure 9

Overexpression of α-synuclein attenuates ferroptosis markers triggered by MB exposure. (a) mRNA levels of GPx4 was determined in cells with endogenous and overexpressed α-synuclein in the presence of MB. Results are expressed as fold change compared to the control and represent (). (b) Cellular oxidant levels were determined using the probe H2DCFDA after Fer-1 (6 μM) treatment. Results are expressed as arbitrary units and represent (). (c) Mitochondrial alterations were evaluated using MitoTracker Red staining. Quantification of dye diffusion was determined by the RawIntDen parameter (right) and profile plots (along a line drawn from the middle of the nuclei to the edge of the cell) (down). Results are expressed as arbitrary units and represent (). Scale bar: 20 μm. (a–c) , , and for each condition with respect to the control; one-way ANOVA and Tukey’s post hoc test.

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