Ferroptosis in Rat Lung Tissue during Severe Acute Pancreatitis-Associated Acute Lung Injury: Protection of Qingyi Decoction

Figure 4

QYD enhancing ALDH2 activity protects against ferroptosis in SAP-associated ALI. (a) qRT-PCR was performed to assess the mRNA expression level of ALDH2 in the rat lung tissue of each group. GAPDH was used as the reference gene. (b, c) Western blotting was conducted to evaluate the protein expression levels of ALDH2, GPX4, p65, and p-p65 in the lung tissue of rats. β-Actin was used as a loading control. (d–f) Semiquantification of protein expression of ALDH2, GPX4, and p-p65 using histograms. (g) Measurement of Fe²⁺ in rat lung tissue of each group served as an iron metabolism indicator. (h) MDA concentration in the rat lung tissue of each group was measured as an indicator of lipid peroxidation. (i) GSH levels in rat lung tissue of each group were monitored to reflect antioxidant capacity. Data are presented as representative images or as the of each group of rats ( per group) from at least three separate experiments. .

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