Mitochondrial Dysfunction and Increased DNA Damage in Vascular Smooth Muscle Cells of Abdominal Aortic Aneurysm (AAA-SMC)

Figure 2

Antioxidative response element (ARE) activity was analysed in healthy () and AAA-VSMC () cultures following 3 h of AngII treatment at 0, 10, 100, and 1000 nM concentrations. ARE activity was determined as relative luminescence (RLU) of the ARE-driven NanoLuc luciferase divided by the RLU of a reference firefly luciferase. (a) ARE activity in untreated cells. Data points represent the means of four replicates per culture and are shown with median and range of all cultures. Statistical analysis was performed with the Mann–Whitney test. ns: not significant. (b, c) ARE activity in healthy VSMC (b) and AAA-SMC (c) as a function of 3 h treatment with different doses of AngII. Data points represent the means of four replicates per culture and are shown with median. Ordinary one-way ANOVA was performed to test for a linear trend. . The dotted line represents the trend line.

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