Curcumin Induces Ferroptosis in Follicular Thyroid Cancer by Upregulating HO-1 Expression

Figure 3

Curcumin inhibits FTC tumorigenesis. (a) Nthy-ori-3-1 and FTC cells were treated with the indicated concentrations of curcumin for 24 h, and curcumin inhibited cell viability. Cell viability was measured using CCK-8 assays. (b–d) Curcumin suppressed the growth of FTC cells. FTC cells were treated with 0, 10, or 20 μM curcumin and subjected to colony formation, cell migration, and invasion assays. Scale bars: 100 μm. and compared with the 0 μM curcumin group. (e, f) FTC cells were either preincubated with various inhibitors, namely, ferrostatin-1 (Fer-1, 10 μM), deferoxamine (DFO, 10 μM), chloroquine (CQ, 5 μM), autophagy inhibitor (3MA, 1 mM), and Z-VAD-FMK (5 μM), for 2 h and then treated with 10 or 20 μM curcumin (Cur) for 24 h or treated with curcumin alone. Cell viability was assessed using CCK-8 assays. and compared with the group receiving curcumin treatment alone. The data are presented as the (SEM), . One-way ANOVA (b–d) and test (e, f) were used to determine statistical significance.

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