Curcumin Induces Ferroptosis in Follicular Thyroid Cancer by Upregulating HO-1 Expression

Figure 6

HO-1 protein interacts with GPX4 protein and promotes ferroptosis in FTC cells. (a) The interaction between HO-1 and GPX4 was confirmed by Co-IP assays, followed by western blotting. (b, c) Relative HO-1/GPX4 mRNA expression in FTC cells transfected with siHO-1 or siHO-1 + siGPX4. The expression levels of mRNA were normalized to the level of β-actin. compared with the Nc siRNA group. ^## compared with the siHO-1 group. (d, e) Determination of lipid ROS levels via fluorescence intensity measurements in FTC cells transfected with siHO-1 or siHO-1 + siGPX4 after treatment with 20 μM curcumin; scale bars: 50 μm. compared with the siHO-1 + curcumin group. The data are presented as the (SEM), . One-way ANOVA (b–e) was used to determine statistical significance.

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