Research Article
NF-κB Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner
Figure 2
Myrislignan regulated the expression of EMT-related genes and inhibited the phosphorylation of p65 protein. (a–d) The protein (E-cadherin, Snail1, and Slug) expression was detected via western blot assay after myrislignan treatment in U87 and U251 cells. (e–j) The protein (total p65/IκB-α and phosphorylated p65/IκB-α) levels were measured via western blot assay after myrislignan treatment in U87 and U251 cells. (k) Immunofluorescence of p65 and p-p65 protein after myrislignan treatment in U87 cells. (l, m) Dual-luciferase reporter assay was conducted to evaluate the status of NF-κB signal in GBM cells treated with myrislignan. Scale bar, 10 μm. ; ; ; ns: no significance.
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