RING Finger Protein 10 Regulates AP-1/Meox2 to Mediate Pirarubicin-Induced Cardiomyocyte Apoptosis

Figure 5

THP causes RNF10 protein abnormalities and cardiomyocyte apoptosis in H9C2 cardiomyocytes. (a, b) Immunofluorescence showed that compared with the CON group, THP caused a decrease in RNF10 expression, while RNF10 expression in the RNF10 silencing group also decreased; compared with that in the THP group, the expression of RNF10 in the RNF10 overexpression group was significantly increased. (c, d) WB results showed that compared with the CON group, THP and RNF10 silencing resulted in decreased expression of RNF10, Meox2, and Bcl-2/Bax proteins, while the expression of AP-1 and cleaved caspase-3 proteins increased; (e, f) compared with the THP group, overexpression of RNF10 resulted in increased expression of RNF10, Meox2, and Bcl-2/Bax protein, while the expression of AP-1 and cleaved caspase-3 protein decreased. Values are expressed as the . One-way ANOVA (). vs. ND, ^# vs. THP.

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