Evaluation of Antidiabetic Effect of Combined Leaf and Seed Extracts of Moringa oleifera (Moringaceae) on Alloxan-Induced Diabetes in Mice: A Biochemical and Histological Study

Figure 12

Histological sections of the pancreas in the experimental groups. (a) Pancreatic tissue of nondiabetic control mice (G1) showing the normal histological structure of the pancreas parenchyma; pancreatic lobules are surrounded by connective tissue capsules, excretory ducts, regular islets of Langerhans, and secretory acini. (b) Pancreatic tissue of diabetic control mice (G2) showing pancreatic histopathological changes that include irregular pancreatic acini tissue, severe shrinkage and necrosis of islet of Langerhans with fatty infiltration in the pancreas parenchyma, severe congestion of blood vessels, and vacuolations of pancreatic acini, disrupted outlining of islets of Langerhans, and dilated intralobular ducts. (c) Insulin-treated diabetic mice (G3) showing mild pancreatic histopathological changes. (d, e) Pancreatic tissue of diabetic mice treated with 500 mg/kg BW/day Moringa oleifera leaf (G4) and seed (G5) extracts for 1 month with persistent histopathological changes: shrinkage in the islets of Langerhans and less fatty infiltration. (g–i) Pancreatic tissue of diabetic mice treated with extracts (500 mg/kg BW/day) of Moringa oleifera leaf (G7), seed (G8), and combination (G9) extracts for 3 months, showing the significant recovery of pancreatic histopathological changes, especially in G6 and G9 (f, i), which show the normal arrangement of pancreatic acini and islets of Langerhans, similar to the nondiabetic control group (G1) (hematoxylin and eosin staining, 400x magnification).