Substance P-Mediated Vascular Protection Ameliorates Bone Loss

Figure 1

Ovariectomy (OVX) causes bond loss and the lack of type H vessel. (a) Change of rat body weight at 0, 4, and 8 weeks post-OVX induction. (b, c) Micro-CT analysis for quantification of bone density was determined at 0, 4, and 8 weeks postinduction. (d) Schematic diagram of microenvironment of bone metaphysis region. (e) The representative images of Masson’s Trichrome (MTC). Expression of CD31 (f) and endomucin (g) in femoral metaphysis regions was determined by IHC staining. The dotted lines indicate boundary between growth plate and metaphysis. Scale bar: 100 μm. (h, i) Quantification for intensity of CD31 and endomucin staining was performed. (j, k) Western blot analysis of CD31 expression in BMA at 0, 4, and 8 weeks postinduction was performed. GAPDH was used as internal loading control and normalized for quantitative analysis. Values are deviation (SD), , and . BV: trabecular bone volume; TV: tissue volume; BS: bone surface; n.s.: not significant.

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