Substance P-Mediated Vascular Protection Ameliorates Bone Loss

Figure 4

SP treatment blocks the deficiency of type H vessel from OVX-induced impairment. (a) The representative images of IHC staining for CD31 and endomucin in femoral metaphysis regions. The dotted lines indicate boundary between growth plate and metaphysis. Scale bar: 100 μm. (b, c) Quantification for intensity of CD31 and endomucin staining was performed. (d, e) Western blot analysis for CD31 expression using BMA at 4 week was performed. GAPDH was used as internal loading control and normalized for quantitative analysis. Values are , , .

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