50 Hz Electromagnetic Field Produced Changes in FTIR Spectroscopy Associated with Mitochondrial Transmembrane Potential Reduction in Neuronal-Like SH-SY5Y Cells

Table 1

Integrated areas of representative vibration bands computed for exposed and not exposed samples, whose limits of integration were 3340−3260 cm⁻¹, 2980−2947 cm⁻¹, 2945−2900 cm⁻¹, 2865−2835 cm⁻¹, 1750−1730 cm⁻¹, 1730−1710 cm⁻¹, 1660−1640 cm⁻¹, 1640−1610 cm⁻¹, 1250−1180 cm⁻¹, and 1120−1010 cm⁻¹, respectively. Each value reported was averaged over a number of 26 spectra acquired at successive exposures. The cells of the table without values (n.d.) correspond to no appreciable change between exposed and not exposed samples or to not detected vibration band in the relative spectrum. The exposure levels refers to a time exposure of 4 h. ^∗𝑃<,0.05, ^∗∗𝑃<0.005 and ^∗∗∗𝑃<0.001 significant differences in comparison to control cells.

Exposure levels; (mT)	Amide A 3295 cm^−1	asCH3 2961 cm^−1	asCH2 2925 cm^−1	sCH2 2853 cm^−1	1740 cm^−1	1725 cm^−1	β-Sheet —1635 cm^−1	α-Helix —1652 cm^−1	^asPO2^− 1235 cm^−1	^sPO2^− 1080 cm^−1
0.00	1.00±0.1	1.00±0.07	1.00±0.1	1.00±0.1	1.00±0.08	1.00±0.1	1.00±0.09	1.00±0.1	1.00±0.07	1.00±0.07
0.050	0.67±0.05	1.15±0.06	1.26±0.04	1.76±0.03	1.55±0.10	0.57±0.05	n.d.	n.d.	n.d.	n.d.
0.81	0.32±0.04	1.54±0.15	1.84±0.07^∗∗∗	2.02±0.08^∗∗∗	2.03±0.15^∗	1.25±0.10	1.29±0.05	1.13±0.04	0.78±0.07^∗	0.64±0.06^∗
1.41	0.64±0.04	1.67±0.20^∗∗∗	2.69±0.15^∗∗∗	3.08±0.15^∗∗∗	2.88±0.07	1.14±0.04	1.95±0.10	1.31±0.08	n.d.	n.d.