Role of Heparan Sulfate 2-O-Sulfotransferase in Prostate Cancer Cell Proliferation, Invasion, and Growth Factor Signaling
Figure 2
Inhibition by 2OST siRNA decreases prostate cancer cell proliferation and migration. (a) BrdU incorporation in LNCaP, C4, C4-2, and C4-2B cell lines. The cell lines are listed in order of increasing metastatic potential from left to right on the graph. All samples were normalized to controls (scrambled siRNA treated). Black bars represent control samples transfected with scrambled siRNA. White bars represent samples transfected with 2OST siRNA. Error bars represent independent samples and standard deviation. Asterisk indicates . (b) Western blot for verification of 2OST siRNA. Levels of 2OST in each sample were normalized to actin by densitometry. Values are representative of two independent experiments. (c) Inhibition by 2OST siRNA decreases Matrigel invasion by C4-2B cells. The number of C4-2B cells that migrated through Matrigel was counted for control cells (scrambled siRNA treated) and experimental cells (2OST siRNA treated) in four separate fields in three independent experiments. The same experiment was performed with control inserts. The average number of invading cells () was normalized to the average number of cells on control inserts () to determine percent invasion. Average % invasion for control and 2OST siRNA cells were and , respectively (, asterisk). (d) Representative images of C4-2B cells used in Matrigel assay. Cells that migrated through the matrigel in either control or 2OST siRNA samples are shown in (a,b).Cells used in control inserts are shown on (c,d).
