Role of Heparan Sulfate 2-O-Sulfotransferase in Prostate Cancer Cell Proliferation, Invasion, and Growth Factor Signaling
Figure 7
Inhibition of NFκB by siRNA results in decreased 2OST mRNA. (a) Schematic representation of 2OST promoter with regions of predicted NFκB-binding sites (N1 and N2). (b) Inhibition of NFκB by siRNA results in decreased levels of 2OST mRNA. Real-time PCR analysis of NFκB and 2OST normalized to levels of 18S. Black bars represent samples treated with scrambled siRNA treated samples and gray bars represent either NFκB or 2OST levels in NFκB siRNA treated cells. Error bars indicate standard deviation. Asterisk indicates .(c)NFκB does not bind directly to the 2OST promoter at predicted binding sites in C4-2B. Chromatin immunoprecipitation analysis of C4-2B total chromatin, NFκB IP, no antibody (NoAB), and Mock samples. Samples were analyzed by PCR with primers flanking each predicted NFκB site. Positive control primers were used from the PPM1D promoter [37].
