Histone Deacetylase Inhibitors Restore Cell Surface Expression of the Coxsackie Adenovirus Receptor and Enhance CMV Promoter Activity in Castration-Resistant Prostate Cancer Cells
Analysis of GFP expression by fluorescence microscopy and quantification by flow cytometry. LNCaP (a) and C4-2b (b, c) were plated overnight and exposed to 10 MOI AdGFP in the absence (a, b) or presence of (c) 10 μM MS-275. Fluorescent images were collected using identical camera settings 48 hrs after viral transduction (magnification = 200x). (d) Quantification of GFP-positive cells by flow cytometry 24 hrs after viral transduction in the absence or presence of 100 ng/mL romidepsin or 10 μM MS-275. Data shown are the mean ± SD from three experiments.
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