Prostate Cancer / 2012 / Article / Fig 7

Research Article

Histone Deacetylase Inhibitors Restore Cell Surface Expression of the Coxsackie Adenovirus Receptor and Enhance CMV Promoter Activity in Castration-Resistant Prostate Cancer Cells

Figure 7

The effect of HDACi on gene expression in 22Rv1 cells. (a) 22Rv1 cells were transduced with 10 multiplicities of infection with AdGFP in the absence or presence of 250 ng/mL Romidepsin or 1 μM MS-275. Fluorescent images were collected 24 hrs after viral transduction (magnification = 200x). (b) 22Rv1 cells were transfected with pCMV.luc. The luciferase signal was quantified 48 hrs after transfection in the absence or presence of 100 ng/mL romidepsin (RD) or 1 μM MS-275. The photograph is a false color image of three representative wells indicating relative numbers of photons produced during a timed interval after luciferin addition. Red indicates more photons; blue indicates less photons.

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