Histone Deacetylase Inhibitors Restore Cell Surface Expression of the Coxsackie Adenovirus Receptor and Enhance CMV Promoter Activity in Castration-Resistant Prostate Cancer Cells
The effect of HDACi on gene expression in 22Rv1 cells. (a) 22Rv1 cells were transduced with 10 multiplicities of infection with AdGFP in the absence or presence of 250 ng/mL Romidepsin or 1 μM MS-275. Fluorescent images were collected 24 hrs after viral transduction (magnification = 200x). (b) 22Rv1 cells were transfected with pCMV.luc. The luciferase signal was quantified 48 hrs after transfection in the absence or presence of 100 ng/mL romidepsin (RD) or 1 μM MS-275. The photograph is a false color image of three representative wells indicating relative numbers of photons produced during a timed interval after luciferin addition. Red indicates more photons; blue indicates less photons.
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