Research Article

Analysis of Argonaute Complex Bound mRNAs in DU145 Prostate Carcinoma Cells Reveals New miRNA Target Genes

Figure 3

Response of 3′UTRs towards miRNAs. The DEDD (a), TNFRSF10B (b), TP53INP1 (c), SPARC (d), ABI2 (e), and PRDX3 (f) 3′UTRs were cloned behind the luciferase reporter gene of the pMIR vector. The reporter gene constructs were expressed with the miRNA expression construct or with the empty pSG5 vector in the indicated combinations. Results represent the mean of 4 independent experiments performed in duplicate. .
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