Analysis of Argonaute Complex Bound mRNAs in DU145 Prostate Carcinoma Cells Reveals New miRNA Target Genes

Figure 4

Identification of miRNA binding sites. The potential binding sites for the indicated miRNAs in the 3′UTRs of DEDD (a), TNFRSF10B (b), TP53INP1 (c), and SPARC (d) were mutated by site directed mutagenesis (mut) and coexpressed with the miRNA expression construct or with the empty pSG5 vector in the indicated combinations. Results represent the mean of 4 independent experiments performed in duplicate. .

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