Interleukin-10 Induces Expression of Neuroendocrine Markers and PDL1 in Prostate Cancer Cells

Figure 1

IL10 induction of morphological changes and expression of NSE and SYP neuroendocrine markers in PCa cells. PCa cells grown in vitro were stimulated with 100 ng/ml IL10, 100 ng/mL IL6, or 10 µM ENZ for 7 days. The morphological changes induced by different treatments in LNCaP, 16D^CRPC, and 42D^ENZR resistant cells were imaged using a light microscope (a). The expression levels of NSE or SYP were determined by immunoblotting of the cell lysates in LNCaP (b), 16D^CRPC (c), and 42D^ENZR resistant (d) cells. Data plotted represents NSE and SYP band intensities normalized to GAPDH protein levels. The statistical significance for the difference between untreated (Un.) and different treatment was determined by one-way ANOVA test with Tukey’s correction. , , , and . Data are representative of three independent experiments.

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