Carriage of Ser217Leu and Ala541Thr Variants of ELAC2 Gene and Risk Factors in Patients with Prostate Cancer in Burkina Faso

Figure 1

Photographs of agarose gels showing PCR-RFLP profiles of Ser217Leu and Ala541Thr polymorphisms of the ELAC2 gene. (a) Ser217Leu polymorphism. PCR-amplified DNA fragments containing the Ser217Leu polymorphisms were digested with Taq α I and then subjected to 2% agarose gel electrophoresis. The TT mutant genotype generates one DNA fragment of 276 bp, the CT genotype generates three DNA fragments of sizes 276 bp, 172 bp, and 104 bp, and the CC wild type generates two DNA fragments of sizes 172 bp and 104 bp. (b) Ala541Thr polymorphism. PCR-amplified DNA fragments containing the Ala541Thr polymorphisms were digested with Fnu4HI and then subjected to 2% agarose gel electrophoresis. The AA mutant genotype (not found in the population), reportedly generated DNA fragments of 165 bp, the GA genotype generated three DNA fragments of sizes 165 bp, 110 bp, and 55 bp, and the GG wild type generated DNA fragments of sizes 110 bp and 55 bp.

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