PINK1-Interacting Proteins: Proteomic Analysis of Overexpressed PINK1

Figure 2

Specificity of an anti-LRPPRC antibody. Fibroblasts were incubated with PINK1 siRNA, LRPPRC siRNA, or scrambled siRNA for 24 h. Whole cell lysates were analyzed by Western blotting with an antibody against LRPPRC. LRPPRC levels decreased only when LRPPRC siRNA was employed, confirming the specificity of the anti-LRPPRC antibody used in our study. The mitochondrial markers HSP60 and VDAC1 served as loading controls. HSP60: Heat shock 60 kDa protein; LRPPRC: Leucine-rich PPR motif-containing protein; VDAC1: Voltage-dependent anion channel 1.