Models for LRRK2-Linked Parkinsonism

Table 2

LRRK2 potential interaction proteins.

Pathway	LRRK2 fragment	Link with LRRK2	Method	References
Apoptosis	Full length	FADD	Co-IP; (HEK293T cells, mouse brain)	[93]
	Full length	TRADD, RIP1	Co-IP; (HEK293T cells)	[93]
Synaptic vesicle endocytosis	Full length; LRR	Rab5b	YTH, pulldown,; Co-IP	[111]
MAPK signaling	Full length,; COR,; Kinase domain	MKK3	Co-IP; (HEK293T cells)	[72, 73]
	Full length	MKK4	Co-IP; (HEK293T cells)	[72]
	Full length,; COR,; Kinase domain	MKK6	Co-IP; (HEK293T cells), C. elegans	[72, 73]
	Full length,; COR,; Kinase domain	MKK7	Co-IP; (HEK293T cells)	[72, 73]
	Full length	JIP1–3	Co-IP; (HEK293T cells)	[74]
	Full length	JIP4	Co-IP; (HEK293T cells)	[74]
Chaperone machinery	Full length,; Kinase domain,; N-term	Hsp90	Co-IP; (HEK293T cells, mouse brain),; YTH	[81, 88, 97–99]
	Full length,; Kinase domain,	p50^CDC37	Co-IP; (HEK293T cells, mouse brain)	[38, 81]
	Full length,; ROC, N-term	CHIP	Co-IP; (HEK293T cells, mouse brain),; YTH	[98, 99]
cytoskeleton	Full length,; ROC	a-tubulin	pulldown	[102]
	Full length,; ROC	b-tubulin	Co-IP; (HEK293T cells, mouse brain), pulldown	[102, 105]
	Full length	EF1A	Co-purification (insect cells),; Co-IP; (HEK293T cells)	[113]
	—	moesin	in vitro, in vivo	[59, 101]
	Full length,; ROC-COR	DVL1/2/3	YTH, Co-IP; (HEK293T cells)	[109]
	Full length	Sgg/GSK3b	Drosophila	[114]
	Full length	Actin cytoskeleton proteins	QUICK,; Co-IP (NIH3T3 cells)	[115]
Protein translation	—	4E-BP	in vitro, Drosophila	[71, 116]
PD related proteins and others	Full length, COR	Parkin	Co-IP; (HEK293T cells, SH-SY5Y cells, primary neurons),	[79]
	Full length	14-3-3 isoforms	Co-IP; (HEK293T cells, Swiss 3T3 cells, mouse brain, kidney, spleen)	[64–66, 80]

FADD: Fas-associated protein with death domain; TRADD: tumor necrosis factor receptor type 1-associated death domain protein; LRR: leucine-rich repeat; YTH: yeast two-hybrid; ROC: Ras of complex protein; COR: C-terminal of ROC; MKK: mitogen activated protein kinase kinase; JIP: JNK interacting protein; Hsp: Heat shock protein; CHIP: C-terminus of Hsp70 interacting protein; EF1A: elongation factor 1𝛼; DVL: dishevelled family of proteins; Sgg: glycogen synthase kinase 3β homolog Shaggy; QUICK: quantitative immunoprecipitation combined with knockdown.