Effects of Human Alpha-Synuclein A53T-A30P Mutations on SVZ and Local Olfactory Bulb Cell Proliferation in a Transgenic Rat Model of Parkinson Disease
(a) Transgene construction. The transgene construct pUTHTV hm2α-Syn is composed of double mutated form of human α-syn (hm2α-SYN) with A30P and A53T mutations under the control of the 9-kb rat tyrosine hydroxylase promoter. (b) There is a significant difference in the ratio odor/H20 between the 2 groups of rats (*, with the odor being from the coconut milk) in the olfaction test. The WT rats spent more time in the corner with the coconut milk filter paper as compared to the corner with the distilled water (ratio superior to 2). Transgenic animals spent less time in the corner with the coconut milk as a ratio of 1 corresponds to the same period of time spent in both corners. No significant ratio difference was observed between 6, 9, and 14 months for both groups of animals. (c) There is no significant difference in lateral stepping performance between WT and Tg animals indicating that no motor deficits in 18-month old Tg animals.
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