A Possible Novel Anti-Inflammatory Mechanism for the Pharmacological Prolyl Hydroxylase Inhibitor 3,4-Dihydroxybenzoate: Implications for Use as a Therapeutic for Parkinson’s Disease
Figure 3
BV2 cells were pretreated with DHB for 1 h (50 & 100 μM) and then stimulated with LPS (100 ng/mL) for a 1 h incubation period. Cells were lysed, run on a SDS-PAGE gel, transferred to PVDF membranes, and blotted with specific antibodies to (a) phosphorylated p65 NFκB subunit or (b) phosphorylated or unphosphorylated p38 and JNK. Actin was used as a loading control. Band density (integrated density value) is expressed graphically as a percentage ratio of densitometric optical density of phosphorylated forms to that of nonphosphorylated p38 and JNK. Data (mean ± SD) are from three independent experiments; * relative to untreated control sample.
