A Possible Novel Anti-Inflammatory Mechanism for the Pharmacological Prolyl Hydroxylase Inhibitor 3,4-Dihydroxybenzoate: Implications for Use as a Therapeutic for Parkinson’s Disease
Figure 4
(a) After pretreatment with DHB (100 μM) for 1 h, BV2 cells were stimulated with 100 ng/mL LPS for 1 h and nuclear translocation of the NFκB p65 subunit assessed by confocal fluorescence microscopy using a fluorescent anti-p65 antibody. Representative laser confocal microcopy images of p65 (red stain) and nuclear DAPI staining (blue) in cells exposed to LPS ± DHB are shown; pink, merged. (b) BV2 cells were transiently transfected with NFκB-Luc for 24 h and treated with 100 ng/mL LPS for 4 h ± DHB for 1 h. Cell lysates were assayed for luciferase activity (mean ± SE, ). * versus control, ** versus LPS.
