Figure 5: Effect of NAC on MA-induced apoptotic cell death in N27 cells. Cells were pretreated with 5 mM NAC for 1 hour followed by treatment with MA (2 mM) or PBS for 24 hours. DNA fragmentation was quantified using a cell death detection using Roche Elisa PLUS kit. The data are expressed as percentage of DNA fragmentation compared with untreated control cells, and asterisks (*** ) indicate significant differences between untreated control group with MA-treated group and MA-treated group with NAC group with MA treatment.