The Protective Effect of Minocycline in a Paraquat-Induced Parkinson's Disease Model in Drosophila is Modified in Altered Genetic Backgrounds
PQ and MC effects on viability are modified by components of stress response pathways. (a) Effect of bsk and Akt loss-of-function mutant alleles on PQ and MQ effects on survival. Treatment with 1 mM MC failed to improve the survival of bsk and Akt loss-of-function mutants fed 10 mM PQ. and each data point represents at least three independent replications of 40 flies for each.genotype. (b) Effect of 1 mM PQ and 1 mM MC on loss-of-function mutants of signaling pathways, JNK, Akt, reaper, caspase, and ERK. rolled (ERK), reaper and caspase mutants showed an extension of life span with MC treatment, while the survival of JNK and Akt mutants was unmodified in the presence of MC. ** = represents significant difference between PQ and PQ with MC groups. ## = and shows a significant difference between PQ-fed wild type and PQ-fed bsk and Akt loss-of-function mutants. . $$ = represents significant difference between PQ-fed wild type and PQ-fed caspase loss-of–function mutant. Error bars represent standard error of the mean. and each data point represents at least three independent replications of 50–60 flies each.