17-Ethynyl-androst-5-ene-3,7,17-triol (HE3286) Is Neuroprotective and Reduces Motor Impairment and Neuroinflammation in a Murine MPTP Model of Parkinson’s Disease
HE3286 attenuated TH-positive neuron loss in the SNpc. Mice (male, C57/Bl6, 8 per group) were given four injections of 20 mg/kg MPTP 2 h apart. HE3286 (40 mg/kg), vehicle, or saline was administered by oral gave twice daily for four consecutive days beginning 1 h after the last MPTP injection. Four days after MPTP injection, mice were sacrificed and brains collected. Coronal SNpc sections from four randomly selected animals per group were immunoreacted with anti-TH antibodies. TH positive neurons were determined on three serial sections per animal. *Significantly greater compared to vehicle, P = 0.003; significantly lower compared to sham, P = 0.0024, by the Mann-Whitney exact test.