Magnolol Protects against MPTP/MPP+-Induced Toxicity via Inhibition of Oxidative Stress in In Vivo and In Vitro Models of Parkinson’s Disease
Figure 3
Effect of post-MPTP treatment with magnolol on DAT, TH, and GFAP protein levels in the striatum of C57BL/6 mice. Mice were received a single injection of 40 mg/kg MPTP and administered daily with magnolol (30 mg/kg) for 4 days at the day just after MPTP treatment. The evaluations were performed at 1 h after the last treatment with magnolol. Representative immunoblots of DAT, TH, and GFAP protein in the striatum are shown (a). Actin protein was used as house keeping protein. Immunoblots of DAT (b), TH (c), and GFAP (d) in the striatum were quantified in each group. Densitometric analysis of protein bands was performed using software (Image J 1.36, NIH). The data were expressed as % of control. Results are means ± SD (-4) from three independent experiments. Statistical comparisons were carried out by one-way ANOVA followed by Tukey test. *** versus control, # versus MPTP + vehicle.
