Dopamine Cytotoxicity Involves Both Oxidative and Nonoxidative Pathways in SH-SY5Y Cells: Potential Role of Alpha-Synuclein Overexpression and Proteasomal Inhibition in the Etiopathogenesis of Parkinson's Disease
Figure 3
Measurement of oxidative stress parameters. (a) Effect of NAC (2.5 mM) on DA (400 μM)-induced production of hydrogen peroxide (H2O2) in SH-SY5Y cells: production of H2O2 was measured during in vitro incubation of SH-SY5Y cells (control, 400 μM DA treated and 400 μM DA + 2.5 mM NAC treated) in Krebs-Ringer’s buffer as detailed in Section 3. Values (expressed as nmol of H2O2 per mg protein) are the means ± SD of four observations. Statistically significant differences exist ( = 132.63, )) between the DA-treated groups and control, as well as between the DA-treated groups and NAC-treated groups, as shown by asterisks (*). (b) Measurement of protein bound quinones in SH-SY5Y cells: levels of protein bound quinones were measured in SH-SY5Y cells incubated without or with DA (400 μM) for 24 h in the presence or absence of NAC (2.5 mM) as detailed in Section 3. Values (optical density at 530 nm (OD 530) per mg protein) are the means ± SD of four observations. Statistically significant differences exist ( = 35.79, ) between the DA-treated groups and control, as well as between the DA-treated groups and NAC-treated groups, as shown by asterisks (*).
