Dopamine Cytotoxicity Involves Both Oxidative and Nonoxidative Pathways in SH-SY5Y Cells: Potential Role of Alpha-Synuclein Overexpression and Proteasomal Inhibition in the Etiopathogenesis of Parkinson's Disease
Figure 6
Lactacystin effects on mitochondrial functions in SH-SY5Y cells. SH-SY5Y cells were exposed to lactacystin (5 μM) for 24 h followed by the measurement of (a) mitochondrial membrane potential and (b) intracellular ATP content, as described in Section 3. Values are the means ± SD of six observations. Student’s -test, paired, shows significant difference (*) in the mitochondrial membrane potential between the lactacystin-treated group (M = 8.39, SD = 0.71) and the control group (M = 13.25, SD = 1.22); (5) = 12.81 (a). Similarly, a significant difference () in the ATP content between the lactacystin-treated group (M = 71.79, SD = 10.08) and the control group exists; (5) = 3.79 (b).
