Dopamine Cytotoxicity Involves Both Oxidative and Nonoxidative Pathways in SH-SY5Y Cells: Potential Role of Alpha-Synuclein Overexpression and Proteasomal Inhibition in the Etiopathogenesis of Parkinson's Disease
Figure 8
DA effects on α-synuclein gene expression in SH-SY5Y cells. SH-SY5Y cells were incubated alone (control) or with DA (400 μM) or 400 μM DA plus 2.5 mM NAC for 24 h. Cellular RNA was isolated and quantitative RT-PCR was carried out for α-synuclein gene as detailed in Section 3. Results expressed as the fold increase with respect to the control are the means ± SD of six observations. Statistical significance collected by Student’s -test, paired, shows significant difference (*) in the fold of alpha-synuclein gene expression between the DA-treated group (M = 2.73, SD = 1.09) and the NAC co-treated group (M = 1.57, SD = 0.34); (5) = 3.05.
