Cortex Fraxini (Qingpi) Protects Rat Pheochromocytoma Cells against 6-Hydroxydopamine-Induced Apoptosis

Figure 4

The effects of QP water extract on the changes of intracellular ROS caused by 6-OHDA. The PC12 cells were subjected to DCFH-DA (20 M) for 1 h followed by 2 h pretreatment with QP water extract (0.5 mg/mL), and then the PC12 cells were exposed to 500 M 6-OHDA for 2 h before detection of DCF fluorescent intensities. Data were expressed as fold increases of control with means SEM of three separate experiments; versus control and versus 6-OHDA alone group.