Cortex Fraxini (Qingpi) Protects Rat Pheochromocytoma Cells against 6-Hydroxydopamine-Induced Apoptosis

Figure 8

QP water extract exerted neuroprotective effect on 6-OHDA treated PC12 cells through activating PI3-K/Akt/GSK3β pathway. ((a), (b)) The neuroprotective effect of QP water extract on PC12 cells against 6-OHDA can be reversed by LY294002 and in a concentration-dependent manner. PC12 cells were exposed to 500 M 6-OHDA at 2 h after pretreatment with both QP water extract (0.5 mg/mL) and LY294002 and SB415286 at different concentrations as indicated. Data, expressed as percentage of control, were expressed as means SEM of three separate experiments; versus control, and versus 6-OHDA alone group, and versus 6-OHDA with pretreatment of QP water extract group. (c) QP water extract had reversed the decline of phospho-PI3-K, phospho-AKT, and phospho-GSK3β caused by 6-OHDA to a certain level and versus 6-OHDA with QP water extract pretreatment group. PC12 cells were pretreated with QP water extract (0.5 mg/mL, 2 h) and then exposed to 6-OHDA (500 M) for 60 min. (d) The bands from three independent experiments were quantified by densitometry and shown as group. Calculated as fold of control, data were expressed as means SEM of three separate experiments; versus control, versus 6-OHDA alone group, and and versus 6-OHDA with QP water extract pretreatment group.

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