Altered Mitochondrial Respiration and Other Features of Mitochondrial Function in Parkin-Mutant Fibroblasts from Parkinson’s Disease Patients

Figure 3

Mitochondrial network analysis of patient-derived and control fibroblasts. Mitotracker Red and live-cell microscopy were used to visualize the mitochondrial network. (a) Representative images of control fibroblasts (left) and patient fibroblasts (right). Scale bars = 20 μm. All images were assessed in regard to the degree of mitochondrial branching (form factor) and degree of mitochondrial elongation (aspect ratio). The distribution of these parameters in grouped patient-derived (P) and grouped control (Ct) fibroblasts are represented on logarithmic scale in boxplots, . (b) Comparison of form factor, which was significantly lower in patient cells than in control cells (). (c) Comparison of aspect ratio, which was similar in patient and control cells (). ; °, outlier; AU, arbitrary units; ; cells analyzed.

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