Methyl-Arginine Profile of Brain from Aged PINK1-KO+A53T-SNCA Mice Suggests Altered Mitochondrial Biogenesis

Figure 1

Workflow chart illustrating the technical approach to quantify the mono-methyl-arginine-modification of peptides throughout the global brain proteome in a quantitative and label-free manner by immunoprecipitation and mass spectrometry. The immunoprecipitation step illustrates the motif antibody (above), the agarose beads (green circle), its immunoglobulin coating (yellow), and the binding of digested peptides (blue) with mono-methyl-arginine modifications (pink). Graphic elements from internet-sites (http://www.cellsignal.com/common/content/content.jsp?id=proteomics-discovery and http://media.cellsignal.com/www/pdfs/proteomics/methylscan_workflow.pdf) were used with permission of Cell Signaling Inc.