Deletion of Herpud1 Enhances Heme Oxygenase-1 Expression in a Mouse Model of Parkinson’s Disease

Figure 1

Expression of Herp after MPTP administration. (a) qRT-PCR. Herpud1^+/+ mice were injected with MPTP, and the total RNA (1 μg), which was extracted from the ventral midbrain, was subjected to qRT-PCR. Each value shown is the mean ± the standard deviation (SD) (), and the value derived from the mice without MPTP injection is designated as one. , compared to mice without MPTP administration. (b) Western blots. Herpud1^+/+ mice were injected with MPTP, and protein samples (30 μg) extracted from the CPu were subjected to western blotting with anti-Herp and anti-β-actin antibodies. Each value shown is the mean ± SD (), and the value derived from the mice without MPTP administration is designated as one. , compared to mice without MPTP administration. (c) Immunohistochemistry. Herpud1^+/+ mice were injected with MPTP and perfused with 4% paraformaldehyde at the indicated times. Brain sections, including sections with the SNpc, were analyzed by immunohistochemistry with anti-Herp and anti-TH antibodies. Arrows indicate Herp expression in the TH-positive dopaminergic neurons after MPTP administration. Scale bars = 30 μm.

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