Deletion of Herpud1 Enhances Heme Oxygenase-1 Expression in a Mouse Model of Parkinson’s Disease

Figure 3

Expression of stress-related genes after MPTP administration. (a) qRT-PCR. (b) Western blots. Herpud1^+/+ and Herpud1^−/− mice were injected with MPTP, and the total RNA (1 μg) or protein samples (30 μg) extracted from the CPu were subjected to qRT-PCR (a) or western blotting () (b). Each value shown is the mean ± SD, and the value derived from Herpud1^+/+ mice without MPTP administration is designated as one. ; , compared to mice without MPTP administration. ^#; ^## between the two genotypes. (c) Immunohistochemistry. Herpud1^+/+ and Herpud1^−/− mice were injected with MPTP and perfused with 4% paraformaldehyde after 24 h. Brain sections were subjected to immunohistochemistry with anti-HO-1 and anti-S100β antibodies. Scale bars = 50 μm (c).

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