Essential Oils May Lead α-Synuclein towards Toxic Fibrils Formation

Figure 5

(a) Chemical structures of α-pinene, limonene, 1,8-cineole, and linalool (left to right, resp.). (b) The α-Syn fibrillation rate in the absence (●) and presence of 2% (v/v) of α-pinene (), limonene (), 1,8-cineole (▲), linalool (○), and the heated oil (□). (c) The fibrillation rate of 2 mg/mL of insulin and (d) HEWL in the absence (black) and the presence of 1% (gray) and 10% (white) (v/v) of M. communis. The proteins were diluted in glycine buffer (30 mM; pH 2.5) and treated with the oil and then incubated for 24 hours at 57°C. The fibrillation rate was measured by ThT fluorescence assay. The chemical structures were drawn by Marvin-Sketch software. (one-way ANOVA) indicated the samples are significantly different from the control.

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