Blood Plasma of Patients with Parkinson’s Disease Increases Alpha-Synuclein Aggregation and Neurotoxicity

Figure 6

SWATCH analysis of α-syn phosphorylation levels following treatment with plasma. Recombinant α-syn was incubated with culture medium containing NS or PD plasma and then purified by immunoprecipitation. Samples were used to identify and quantify protein phosphorylation sites after checking by western blot and ELISA. (a) and (b) Results of western blot and ELISA. (c) MS analysis of phosphorylated peptides. The α-syn samples incubated with PD and NS plasma but not PBS were phosphorylated at Ser129. (d) Quantitative analysis of phosphorylated peptides in o-α-syn-NS and o-α-syn-PD samples. Ser129 phosphorylation increased 6.17-fold in o-α-syn-PD compared with 0.21-fold in o-α-syn-NS samples (/group; one-way ANOVA followed by Tukey’s post hoc test). versus NS group.

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