Comparative mRNA Expression of eEF1A Isoforms and a PI3K/Akt/mTOR Pathway in a Cellular Model of Parkinson’s Disease

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td class="align_left">Gene symbol</td><td class="align_center">Primers sequences 5′-3′</td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">eEF1A1</td><td class="align_center">GGCATACCCGAGAGCATG</td></tr><tr><td class="align_center">AGGCATGTTAGCACTTGGC</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">eEF1A2</td><td class="align_center">GAGCCCTCCCCCAACATGCC</td></tr><tr><td class="align_center">ATGTTCACTGGCGCAAAGGTCAC</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3CA</td><td class="align_center">TGGATGCTCTACAGGGCTTT</td></tr><tr><td class="align_center">GTCTGGGTTCTCCCAATTCA</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3CB</td><td class="align_center">GCATTAAAAGGGAGCGAGTG</td></tr><tr><td class="align_center">CATGCCGTCGTAAAATCAGA</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3CD</td><td class="align_center">CTGGCTGAAGTCCAAGAACC</td></tr><tr><td class="align_center">CTCGGATCATGATGTTGTCG</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3CG</td><td class="align_center">ATACCATGATAGCGCCCTTG</td></tr><tr><td class="align_center">AATCACAGCGAACCTCTGCT</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3C2A</td><td class="align_center">GAAAAACGAGGAATCCGACA</td></tr><tr><td class="align_center">CAGGGTTACTCCACCCAAGA</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3C2B</td><td class="align_center">TTCCCTAGTCGCTTCGTGAT</td></tr><tr><td class="align_center">CAGTGGGTGGAAGAAGGTGT</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3C2G</td><td class="align_center">TTCATCTCCCAGATGGCTCT</td></tr><tr><td class="align_center">AGTGGGGTCCGTACATTTTG</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">PIK3C3</td><td class="align_center">TCAGCCAAGCATTGTTGAAG</td></tr><tr><td class="align_center">TCCACTTTCGCGTTGTACTG</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">Akt1</td><td class="align_center">TCTATGGCGCTGAGATTGTG</td></tr><tr><td class="align_center">CTTAATGTGCCCGTCCTTGT</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">Akt2</td><td class="align_center">TATACCGCGACATCAAGCTG</td></tr><tr><td class="align_center">GGTCCCACAGAAGGTTTTCA</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">Akt3</td><td class="align_center">TGGACAAAGATGGCCACATA</td></tr><tr><td class="align_center">ACTGCTCGGCCATAGTCATT</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">mTORC1</td><td class="align_center">AGGCCGCATTGTCTCTATCAA</td></tr><tr><td class="align_center">GCAGTAAATGCAGGTAGTCATCCA</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">mTORC2</td><td class="align_center">TCTACCACGACAGCCCGGCA</td></tr><tr><td class="align_center">TGGGGGCCCCGTTCCATCAT</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="2">β-Actin</td><td class="align_center">CATGTACGTTGCTATCCAGGC</td></tr><tr><td class="align_center">CTCCTTAATGTCACGCACGAT</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

List of primer pairs used for real-time quantitative PCR.

Parkinson’s Disease

tab1

Table 1

Table 1: Comparative mRNA Expression of eEF1A Isoforms and a PI3K/Akt/mTOR Pathway in a Cellular Model of Parkinson’s Disease 