Sirtuin-2 Protects Neural Cells from Oxidative Stress and Is Elevated in Neurodegeneration

Figure 1

The effect of SIRT2 overexpression and inhibition was determined in toxin treated SH-SY5Y cells. SIRT2 was overexpressed in SH-SY5Y cells and control cells were transfected with empty vector following which one set of cells was treated with toxin alone and another with SIRT2 inhibitor AGK2 and toxin for 20 hours and viability measured by reduction of Alamar Blue. Toxins used: diquat (20 µM or 10 µM) or rotenone (20 µM or 0.5 µM) treated cells. Data are presented as fold- untreated (±SD) from three independent assays (). when compared to 0.2% vehicle (PBS or DMSO), one-way ANOVA (Bonferroni corrected), and when compared to control cells, when compared to control + AGK2 treatment, and and when compared to SIRT2 cells, two-way ANOVA (Bonferroni corrected).