Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H₂O₂-Induced Oxidative Damage to MES23.5 Cells

Figure 1

The damage effects of H₂O₂ on MES23.5 cells and the protective effects of KTF against oxidative stress were evaluated using MTT assay. (a) Effects of different concentrations of H₂O₂ treatment on cell damage. MES23.5 cells were treated with 12.5, 25, 50, 100, 150, 200, 300, 400, and 500 μMol/L H₂O₂ for 12, 24, and 48 h. (b) Protective effects of different concentrations of KTF treatment on H₂O₂-induced cell damage. Treatment of KTF resulted in increase of MES23.5 cells viability after H₂O₂ addition. MES23.5 cells were incubated with GE (12.5 μg/ml) or KTF (3.125, 6.25, 12.5 μg/ml) for 24 h after the addition of H₂O₂. The cell viability was measured with MTT. Data are expressed as mean ± SD (standard deviation) in three independent experiments. compared to the control group. compared to the H₂O₂-treat group.

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