Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP⁺-Treated MES 23.5 Dopaminergic Cells

Figure 5

Protein expression of p-JAK2, p-STAT3, and p-ERK1/2 in MES23.5 cells and MPP⁺-treated MES23.5 cells. ((a) and (b)) MES23.5 cells were pretreated with AG490 (JAK inhibitor) or PD98059 (ERK1/2 inhibitor) for 1 hour, followed by CRJ (250 μg/mL) incubation for 24 hours. CRJ alone enhanced the expression of (a) p-JAK2 and p-STAT3 and (b) p-ERK1/2 in MES23.5 cells. The effect could be inhibited by the corresponding pharmacological inhibitors. ((c) and (d)) Time courses of the changes of protein levels of p-JAK2, p-STAT3, and p-ERK1/2 in MPP⁺-treated MES23.5 cells after the posttreatment of CRJ (250 μg/mL). Densitometric analysis of protein expression was determined. Data were represented as mean ± SEM in three independent experiments. Total form of each of the phosphorylated proteins was used as protein loading control. , compared to MPP+-treated cells 0 min after the posttreatment of CRJ.

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