Antidyskinetic Treatment with MTEP Affects Multiple Molecular Pathways in the Parkinsonian Striatum

Figure 1

The protocol of the experiment. Dopamine depletion was induced by 6-OHDA injections in the medial forebrain bundle (MFB) while the sham group was injected with saline in the MFB. Three weeks later, the rats that exhibited apomorphine-induced rotations exceeding 7 turns/min in the apomorphine induction test were put to the subsequent experiment. The sham and PD groups were injected with saline once daily; the LID group received L-Dopa (25 mg/kg) and benserazide (6.25 mg/kg) cocktail once daily, while the MTEP group received MTEP (5 mg/kg) 30 min before the injection of L-Dopa and benserazide. All treatments were performed for 14 days; behavior tests like AIM test, open field test, and cylinder test were performed on days 2, 5, 12, and 14. Two hours after the last injection on the 14th day, all groups were sacrificed for western blot and Q-PCR.