Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease

Figure 1

Establishment of the OxiDJ-1 ELISA. (a) Western blot analysis of OxiDJ-1. To generate OxiDJ-1, His-DJ-1 was incubated with 5 mM H₂O₂ or equivalent vehicle (sterile DW) for 1.5 h at 37°C and subjected to the Western blot analysis. (b) A quantitative analysis using a densitometer of the Western blot results in high molecular weight (HMW), or monomer was indicated by closed bracket or arrowhead, respectively. (c) A schematic diagram representing our OxiDJ-1 ELISA. (d) The standard curve obtained from the OxiDJ-1 ELISA. The ELISA was performed with an oxidized DJ-1 antibody as a capture antibody and HRP-conjugated DJ-1 antibody as a detection antibody using His-DJ-1 pretreated with H₂O₂ or DW as standard proteins.

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