Evaluating the Potential of Portulaca oleracea L. for Parkinson’s Disease Treatment Using a Drosophila Model with dUCH-Knockdown

Figure 2

Food intake and antioxidant accumulating abilities of larvae when treated with P. oleracea L. extract. (a) Effects of PWE on the eating ability of third-instar larvae. The control line TH > GFP-IR (w/y; +; TH-GAL4/UAS-GFP-IR) and the dUCH-knockdown line TH > dUCH-IR (w/y; +; TH-GAL4/UAS-dUCH-IR). Population size N = 10 and biological replication n = 5; one-way ANOVA, post hoc test: Tukey, ns: not significant, , and . Error bars represent the standard deviation of data. (b) The relative antioxidant intake based on the amount of food consumed by larvae. Control line (+; +; TH-GAL4/UAS-GFP-IR) and dUCH-knockdown line (+; +; TH-GAL4/UAS-dUCH-IR). Population size N = 10 and biological replication n = 5; one-way ANOVA, post hoc test: Tukey, , and . Error bars represent the standard deviation of data. (c) The relative level of antioxidant accumulation by dUCH-knockdown larvae (R² = 0.9932, dissociation constant: K_d = 2.001 ± 0.437). Error bars indicate the standard error of the mean.

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