7,8-Dihydroxyflavone Protects Nigrostriatal Dopaminergic Neurons from Rotenone-Induced Neurotoxicity in Rodents

Figure 2

Tyrosine hydroxylase immunostaining of substantia nigra (SN) and striatum in the rotenone-induced PD model. (a) Immunofluorescence of tyrosine hydroxylase in the substantia nigra pars compacta (SNpc). Scale bar = 100 μm (up). We found that there were fewer TH-positive neurons in the similar anatomic level sections of SN in the rotenone group than the control group. Compared to the rotenone group, treatment with 7,8-DHF significantly attenuated the loss of dopaminergic neurons in SNpc (a, e). The western blots of TH in SN further reconfirmed the findings (b, c). , , a significant difference between the two indicated groups. The administration of rotenone also resulted in a decrease of TH-positive DA terminals in the striatal region of animals in the rotenone group compared to the 7,8-DHF group (d, f). Scale bar = 100 μm (down). Data were presented as mean ± SEM .

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